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collagenase type ii iv  (MedChemExpress)
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Collagenase Type Ii Iv, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagenase iv  (Worthington Biochemical)
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Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Collagenase Iv, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagenase iv - by Bioz Stars, 2026-09
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collagenase iv  (Thermo Fisher)
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Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
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collagenase iv  (Yeasen Biotechnology)
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Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Collagenase Iv, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+iv/collagenase+iv/pm42277975-55-7-10
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collagenase iv - by Bioz Stars, 2026-09
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collagenase iv  (MedChemExpress)
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MedChemExpress collagenase iv
Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Collagenase Iv, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+iv/Collagenase/pmc13262578-149-20-22
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collagenase iv  (Nordmark Arzneimittel)
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Nordmark Arzneimittel collagenase iv
Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Collagenase Iv, supplied by Nordmark Arzneimittel, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagenase iv  (Servicebio Inc)
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Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Collagenase Iv, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following collagenase-IV treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.

Journal: STAR Protocols

Article Title: Protocol for isolating viable human central nervous system T cells

doi: 10.1016/j.xpro.2026.104464

Figure Lengend Snippet: Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following collagenase-IV treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.

Article Snippet: Collagenase IV , Worthington , Cat# LS004189.

Techniques: RNA Sequencing, Gene Expression, Expressing